Point mutation in the group B streptococcal pbp2x gene conferring decreased susceptibility to beta-lactam antibiotics.
نویسندگان
چکیده
Beta-lactam antibiotics (BLAs) are the first-line agents used against group B streptococci (GBS) infection. A clonal set of four independent, invasive GBS isolates with elevated MICs to BLAs were identified that shared a pbp2x mutation (Q557E) corresponding to a resistance-conferring pneumococcal mutation. BLA sensitivity was restored through allelic replacement or complementation with the wild-type pbp2x.
منابع مشابه
Point Mutation in the Group B Streptococcal pbp2x Gene Conferring Decreased Susceptibility to -Lactam Antibiotics
Point Mutation in the Group B Streptococcal pbp2x Gene Conferring Decreased Susceptibility to -Lactam Antibiotics Samira Dahesh,† Mary E. Hensler,† Nina M. Van Sorge, Robert E. Gertz, Jr., Stephanie Schrag, Victor Nizet, and Bernard W. Beall* Division of Pharmacology and Drug Discovery, Department of Pediatrics, and Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California...
متن کاملAn important site in PBP2x of penicillin-resistant clinical isolates of Streptococcus pneumoniae: mutational analysis of Thr338.
Penicillin-binding protein 2x (PBP2x) of Streptococcus pneumoniae represents a primary resistance determinant for beta-lactams, and low-affinity PBP2x variants can easily be selected with cefotaxime. Penicillin-resistant clinical isolates of S. pneumoniae frequently contain in their mosaic PBP2x the mutation T338A adjacent to the active site S337, and T338P as well as T338G substitutions are al...
متن کاملبررسی ژن مقاومت بتالاکتاماز CTX در اشرشیا کلی جدا شده از نمونه های عفونت ادراری بیمارستان های آموزشی دانشگاه علوم پزشکی مازندران به روش واکنش زنجیره ای پلیمراز (PCR)
Abstract Background and purpose: Escherichia Coli is one of the most common pathogens associated with nosocomial infection. Increasing use of beta Lactam Antibiotics in treatment of bacterial infections resulted in increments of drug resistance of such bacteria that is caused due to the production of B-lactamase enzymes. The beta lactamase – producing bacteria especially E.coli which is resi...
متن کاملPenicillin-binding proteins 2b and 2x of Streptococcus pneumoniae are primary resistance determinants for different classes of beta-lactam antibiotics.
High-level resistance to beta-lactam antibiotics in Streptococcus pneumoniae is mediated by successive alterations in essential penicillin-binding proteins (PBPs). In the present work, single amino acid changes in S. pneumoniae PBP 2x and PBP 2b that result in reduced affinity for the antibiotic and that confer first-level beta-lactam resistance are defined. Point mutations in the PBP genes wer...
متن کاملGenetic analysis of pbp2x in clinical Streptococcus pneumoniae isolates in Quebec, Canada.
OBJECTIVES To investigate the nature of the amino acid motifs found in penicillin-binding protein (PBP) 2x of penicillin-resistant Streptococcus pneumoniae isolates across the province of Quebec (Canada), and to obtain preliminary information regarding the prevalence of these alterations. METHODS The pbp2x genomic region encompassing codons 178-703, which includes the entire region of the tra...
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 52 8 شماره
صفحات -
تاریخ انتشار 2008